Identification of Italian cypress (Cupressus sempervirens) pollen allergen Cup s 3 using homology and cross-reactivity
نویسندگان
چکیده
منابع مشابه
Quantification assay for the major allergen of Cupressus sempervirens pollen, Cup s 1, by sandwich ELISA.
BACKGROUND The Cupressaceae are an important cause of pollinosis, particularly in Mediterranean countries. Cypress pollen allergenic extracts are difficult to produce since they have a low protein and a high carbohydrate content and consequently accurate standardization of these extracts is essential for diagnosis and immunotherapy. METHOD Natural Cup s 1 was purified by a combination of hydr...
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multi gene family), showed significant sequence and structural similarity to Cyn d 1. This protein was expressed in E. coli and the recombinant protein did not react with serum IgE from grass pollen allergic patients, suggesting that EXPB1 represented a non-allergenic homologue of grass group 1 allergens. It is proposed that differences in the amino acid sequence are responsible for the differe...
متن کاملIgE Reactivity to Common Cypress (C. Sempervirens) Pollen Extracts: Evidence for Novel Allergens
BACKGROUND : Cypress pollen is becoming an increasing cause of respiratory allergy in some regions worldwide. OBJECTIVE : The aim of this study was to determine some of the main allergens implicated in the common cypress (C. sempervirens) pollen allergy. METHODS : Pollen extracts were optimized by using some detergents and chaotropes in order to solubilize both water and non-water soluble p...
متن کاملGuidelines for using pollen cross-reactivity in formulating allergen immunotherapy.
There is a trend among allergen extract companies to diminish the number of individual extracts available for commercial use. This is driven in part by economic considerations and in part by constraints imposed by the US Food and Drug Administration. As much as 2/3 of previously manufactured extracts are or will no longer be available. In this setting, knowledge of pollen allergen interrelation...
متن کاملLinkage mapping of the Mediterranean cypress, Cupressus sempervirens, based on molecular and morphological markers.
Gene mapping for a Cupressus species is presented for the first time. Two linkage maps for the Mediterranean cypress (Cupressus sempervirens) varieties, C. sempervirens var. horizontalis and C. sempervirens var. pyramidalis, were constructed following the pseudo-testcross mapping strategy and employing RAPD, SCAR and morphological markers. A total of 427 loci (425 RAPDs, two SCARs) repres...
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ژورنال
عنوان ژورنال: Annals of Allergy, Asthma & Immunology
سال: 2006
ISSN: 1081-1206
DOI: 10.1016/s1081-1206(10)60798-3